Serological Studies in Bullous Pemphigoid: A Literature Review of Antibody Titers at Presentation and in Clinical Remission

Bullous pemphigoid is associated with antibodies to a 230 kDa and a 180 kDa protein. In a literature review we investigated the role of auto-antibodies as detected by different serological assays. Nine reports containing data on 143 patients were analyzed. Pre-treatment data showed that indirect immunofluorescence, enzyme-linked immunosorbent assay (ELISA) and immunoblotting offer an 82.2% or greater probability of being positive. At the end of the study period, all patients had clinically improved, whether or not they were on therapy. Auto-antibodies were present in 29% of patients evaluated by monkey esophagus immunofluorescence and 75% of those evaluated by human skin immunofluorescence. Positive titers were also reported in 67.6% of patients evaluated by ELISA. In 100% of patients in whom immunoblotting was performed the titers became negative. In 3 patients (5.3%) using human skin immunofluorescence and in one patient (1.4%) using ELISA the titers were increased at the end of the study period. The correlation between anti-basement membrane zone antibodies and the clinical course of bullous pemphigoid requires further and long-term studies

CLINICAL HYPOGLYCEMIC EFFECTS OF ALLIUM CEPA (RED ONION) IN TYPE 2 DIABETIC PATIENTS

Background: Type 2 diabetes mellitus results from defects in insulin secretion and/or insulin action or both. Objectives: The present study was conducted to investigate the hypoglycemic effects of Allium cepa in patients with type 2 diabetes mellitus.   Results: In type 2 diabetic patients (n=21) the administration of crude Allium cepa (100g) markedly reduced fasting blood glucose levels by 40 mg/dl 4 hours later, compared to glibenclamide (81 mg/dl). Also Allium cepa significantly reduced the induced hyperglycemia (GTT) after ingestion of 75 grams dextrose by 159 mg/dl in the test subgroup (n=7) of type 2 diabetic patients to a point below that produced in the negative control group after 4 hours. Conclusion: Crude Allium cepa produced hypoglycemic effects, thus it could be used as a dietary supplement in management of diabetes

The Critical Review of the Performance of the EU Water Framework Directive in Improving the Rivers’ Water Quality and the Impediments in its Implementation

Water is essential in the disposition and growth of living species, especially in the maintenance and survival of human life. History divulges that all the civilisations, countries and big cities were established along the main river courses/ water channels merely because of the importance of water in human life. The increased world population has increased the depletion of this natural resource. Anthropogenic activities pollute water resources by throwing physical, chemical, biological and industrial waste in these streams. The division of regions into states and countries and different ruling bodies in different areas of the world have purported the issues of maintaining control of water bodies and their rights of use within the river basins based on the trans-borders flow/ catchment areas and multinational utilisation. European Union, since its inception, has been issuing different directives regarding the environment, pollution, water usage, ecology and hydrology. In 2000, a detailed directive named “Water Framework Directive (EU WFD 2000)” was formulated by the European Union encompassing significant aspects of all previous directives with the direct responsibility of each member state to arrange implementation of this directive by incorporating this directive in legislation and establishment of independent bodies/ agencies for its implementation in true letter and spirit. Three years were given to incorporate the water framework directive as law. Then further specified periods were given to implement it in a phased manner from 2003 to 2015. Though a tremendous change in attitude towards maintaining the water quality, partial implementation of EU WFD in member states has been achieved, unfortunately; still, the target is too far away, especially in tackling the heavily modified water bodies despite multi-billion investments by public and private sectors. An endeavour has been made to critically review/ evaluate the challenges in implementing the water framework directive and the efforts of member states to overcome these challenges. The scope of this paper is to review some of the available literature on the subject in the form of books, European Union directives, conventions/ conference minutes/ proceedings/ documents, assessment reports, documents of environmental agencies of member states and different presentations followed by a suitable conclusion as per own understanding from literature and assessment by different sources

High fluoride and low pH level have been detected in popular flavoured beverages in Malaysia

Objective: In children, excessive ingestion of fluoride from different sources including bottled drinking water and flavoured beverages or soft drinks can lead to the development of dental fluorosis. In addition, the pH level of beverages is important. Low pH can cause dental erosion. In this study we explore the fluoride content and pH level of certain popular beverages available in Malaysian supermarkets and hawkers' stalls. Methods: Bottled drinking water and selected popular flavoured packet drinks were purchased from a supermarket and the corresponding flavoured hawkers' drinks, from a hawker's stall in Kuala Lumpur. Fluoride and pH of the beverages were determined using digital fluoride meter and digital pH meter respectively. Results: It was found that fluoride content and pH level vary among the beverages. The mean fluoride content in both packet and hawkers' drinks (7.64±1.88 mg/L, 7.51±1.60 mg/L, respectively) was approximately 7 times higher than the bottled drinking water (1.05±0.35 mg/L). Among the beverages, the tea packet drink was found to contain the highest amount of fluoride (13.02±0.23 mg/L). The mean pH of bottled-drinking water was near neutral (6.96±0.17), but acidic for both supermarket (4.78.00±0.49) and hawkers' drinks (5.73±0.24). The lychee packet drink had the lowest pH level (2.97±0.03). Conclusions: Due to the wide variation of the fluoride content and pH level of the drinks tested in this study, it is recommended that steps should be taken to control the fluoride concentration and pH level in beverages if dental fluorosis and erosion are to be prevented. This record was migrated from the OpenDepot repository service in June, 2017 before shutting down

Ameliorative Potential of Morin in Streptozotocin-Induced Neuropathic Pain in Rats

Purpose: To investigate the protective effect of morin, a naturally occurring bioflavonoid of Moraceae family, in experimentally-induced diabetic neuropathy (DN) in rats.Methods: Diabetes was induced by a single injection (65 mg/kg, ip) of streptozotocin (STZ). Morin (15 and 30 mg/kg/day) oral treatment was started 3 weeks after diabetes induction and continued for 5 consecutive weeks. Pain threshold behavior tests were performed at the end of the treatment. In sciatic nerve, inflammatory cytokines (TNF-á, IL-1â, IL-6), nerve growth factor (NGF) and insulin growth factor (IGF-1) were determined using ELISA kits, while thiobarbituric acid reactive substances (TBARS),glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) levels were assessed.Results: Diabetic animals showed apparent decreased paw-withdrawal (39 %, p < 0.05) and tail-flick (31 %, p < 0.05) latency as compared with control group. All the measured biomarkers were altered (p < 0.05 to 0.001) in diabetic rats compared with control non-diabetic animals. Morin treatment attenuated hyperalgesia and analgesia (p < 0.05) respectively. Morin treatment of diabetic rats at both doses significantly decreased the levels of cytokines (p < 0.01), glucose (p < 0.01) and TBARS (p < 0.001), but increased NGF (p < 0.01), IGF-1 (p < 0.01) and GSH (p < 0.01) levels in sciatic nerves compared to untreated diabetic animals. Inhibited activities (U/mg protein) of SOD (1.08 ± 0.16) and CAT (2.77 ± 0.36) in sciatic nerve of diabetic rats also found corrections (2.09 ± 0.11, p < 0.01) and (4.53 ± 0.57, p < 0.01) after morin (30 mg/kg/day) treatment, compared with untreated diabetic animals.Conclusion: These findings demonstrate the protective effect of morin mediated through reduction of oxidative stress and inflammatory process, and suggest the therapeutic potential of morin in the attenuation of diabetic neuropathy.Keywords: Morin, Diabetes, Neuropathy pain, Oxidative stress, Anti-inflammator

Assessment of riverbank filtration performance for climatic change and a growing population

Data availability statement The original contributions presented in the study are included in the article/Supplementary Material; further inquiries can be directed to the corresponding authors.Copyright © 2023 Abd-Elaty, Kuriqi, Ganayem, Ahmed, Saleh and Garrote. Riverbank filtration (RBF) consists of green drinking water production in many regions and is used as a pre-treatment phase. This study investigates the performance of the RBF in the Nile delta, Egypt, for climate change and population growth scenarios of 2030, 2040, and 2050. This study presents a new method for predicting the sharing of riverbanks considering three cases: i) the river stage controlling the water levels in the river, ii) increasing RBF pumping, and iii) changing the groundwater levels. This last scenario is achieved by changing the general head in the MODFLOW model. The results showed that RBF sharing (RBFS) is a proportion of the river leakage inflow, in which the decrease of the river stage due to the influence of climate change reduced the river leakage inflow and RBFS. In addition, increasing RBF pumping, decreasing RBF pumping, and lowering the groundwater levels due to the increase in the future drinking water pumping for the population growth increased the river leakage inflow and RBFS. Finally, combining the three cases decreased RBFS in the coming years of 2030, 2040, and 2050, respectively, due to more groundwater sharing than the river inflow. The results show that the water budget is a good tool to investigate RBFS compared with MT3D results. This technique can reduce the cost of water quality collection and analysis; moreover, it will help with the estimation of RBF and save time compared with solute transport modeling.AK is grateful for the Foundation for Science and Technology’s support through funding UIDB/04625/2020 from the research unit CERIS

Evaluation of biodegradable polyester-co-lactone microparticles for protein delivery.

Abstract Poly(glycerol adipate-co-ω-pentadecalactone) (PGA-co-PDL) was previously evaluated for the colloidal delivery of α-chymotrypsin. In this article, the effect of varying polymer molecular weight (MW) and chemistry on particle size and morphology; encapsulation efficiency; in vitro release; and the biological activity of α-chymotrypsin (α-CH) and lysozyme (LS) were investigated. Microparticles were prepared using emulsion solvent evaporation and evaluated by various methods. Altering the MW or monomer ratio of PGA-co-PDL did not significantly affect the encapsulation efficiency and overall poly(1,3-propanediol adipate-co-ω-pentadecalactone) (PPA-co-PDL) demonstrated the highest encapsulation efficiency. In vitro release varied between polymers, and the burst release for α-CH-loaded microparticles was lower when a higher MW PGA-co-PDL or more hydrophobic PPA-co-PDL was used. The results suggest that, although these co-polyesters could be useful for protein delivery, little difference was observed between the different PGA-co-PDL polymers and PPA-co-PDL generally provided a higher encapsulation and slower release of enzyme than the other polymers tested

Transparent conductive oxide films for high-performance dye-sensitized solar cells

In this paper, atmospheric pressure chemical vapor deposition of fluorine-doped tin oxide (FTO) thin films of various thicknesses and dopant levels is reported. The deposited coatings are used to fabricate dye-sensitized solar cells, which exhibited reproducible power conversion efficiencies in excess of 10%. No surface texturing of FTOs or any additional treatment of dye-covered films is applied. In comparison, the use of commercial FTOs showed a lower cell efficiency of 7.11%. Detailed analysis showed that the cell efficiencies do not simply depend on the resistivity of FTOs but instead rely on a combination of carrier concentration, thickness, and surface roughness properties

Pharyngitis and sore throat: A review

Pharyngitis is a sore throat caused by inflammation of the back of the throat. It is one of the most common reasons for visits to family physicians. Throat may be scratchy and swallowing can be painful. Usually, a sore throat is the sign of another illness, such as a cold or the flu. In this review article, epidemiology, national perspective, regional perspective, pathogenesis, clinical diagnosis, clinical presentation and causes of pharyngitis was described.Key words: Pharyngitis, sore throat, inflammation